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KMID : 0613820120220121637
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Journal of Life Science
2012 Volume.22 No. 12 p.1637 ~ p.1643
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Molecular Mechanisms through Which Peptidoglycan Induces IL-1β Expression in Monocytic Cells
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Seo Hyun-Cheol
Rhim Byung-Yong
Kim Koan-Hoi
Kim Sun-Mi
Lee Sae-A
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Abstract
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This study investigated the effects of PG on IL-1β expression and determined cellular factors involved in PG-mediated IL-1β up-regulation in mononuclear cells in order to understand the molecular mechanisms underlying inflammatory responses associated with bacterial pathogen-associated molecular patterns in the diseased artery. Exposure of human monocytic leukemia THP-1 cells to PG resulted in enhanced secretion of IL-1β and also profound induction of the IL-1β gene transcript. These effects were abrogated by OxPAPC, an inhibitor of TLR-2/4. Pharmacological inhibitors such as U0126, SP6001250, Akti IV, rapamycin, and DPI also significantly attenuated PG-mediated IL-1β up-regulation. However, polymyxin B did not influence the IL-1β expression. This study indicates that PG contributes to vascular inflammation in atherosclerotic plaques by up-regulating expression of IL-1β via TLR-2, Akt, mTOR, MAPKs, and ROS.
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KEYWORD
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Interleukin-1β, peptidoblycan, THP-1 cell
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